human renal proximal tubular epithelial cells hrptepc  (Cell Applications Inc)

Journal: Pharmaceutics

Article Title: Utilizing a Kidney-Targeting Peptide to Improve Renal Deposition of a Pro-Angiogenic Protein Biopolymer

doi: 10.3390/pharmaceutics11100542

Figure Lengend Snippet: In vitro cellular binding/uptake of all constructs in primary human renal cells. Primary human glomerular microvascular endothelial cells (HGME), proximal tubule epithelial cells (HRPTEpC), and podocytes were exposed to each fluorescently labeled ELP fusion protein at 10 µM for 24 h. Protein levels bound to or in each cell type were determined by flow cytometry ( A ). Expression of the VEGF receptors Flt-1 and Flk-1 were determined in each cell line by Western blot (( B ). Lane 1—HGME, Lane 2—HRPTEpC, and Lane 3—podocytes). * Statistically significant increase relative to ELP-treated cells (one-way ANOVA performed within each cell type with post-hoc Tukey’s multiple comparison).

Article Snippet: Human renal proximal tubular epithelial cells (HRPTEpC) were purchased from Cell Applications, Inc. (San Diego, CA, USA) and subcultured according to the manufacturer’s recommendations using RenaEpi Growth factor media.

Techniques: In Vitro, Binding Assay, Construct, Labeling, Flow Cytometry, Expressing, Western Blot, Comparison

Journal: Journal of Cellular and Molecular Medicine

Article Title: y+LAT1 and y+LAT2 contribution to arginine uptake in different human cell models: Implications in the pathophysiology of Lysinuric Protein Intolerance

doi: 10.1111/jcmm.14801

Figure Lengend Snippet: Arginine transport in human renal proximal tubular epithelial cells (HRPTEpC) and in Caco‐2 cells. Panels A and C. HRPTEpC (panel A) and Caco‐2 (panel C) cells, grown in 96‐well plates, were washed in EBSS in the presence or absence of sodium, and arginine transport was measured through a 30 s incubation in the same solution containing [ 3 H]arginine (0.05 mmol/L; 5 μCi/mL) in the absence or presence of 2 mmol/L leucine (+ Leu) or 2 mmol/L leucine+2 mmol/L lysine (+ Leu +Lys) (see ). Bars are mean ± SEM of three independent experiments each performed in quadruplicate. * P < .05, ** P < .01, *** P < .001 vs total uptake; $$ P < .01, $$$ P < .001 vs +Leu. Panels B and D. Data shown in Panels A and C were employed to calculate the relative contribution of systems y + L, b 0,+ and y + (see ). Panel E. For the measurement of arginine transcellular flux in polarized Caco‐2 monolayers, cells, cultured onto inserts, were incubated at the apical side in 100 µL EBSS containing L‐[ 3 H] arginine (0.05 mmol/L; 8 μCi/mL) and at the basolateral side in 550 µL EBSS in the absence or presence of 10 mmol/L leucine (+ Leu). At the times indicated, aliquots of 100 µL of the basolateral medium were collected, replaced with fresh medium and counted for radioactivity. Points are mean ± SD of three independent determinations within a representative experiment that, repeated twice, gave comparable results

Article Snippet: Human renal proximal tubular epithelial cells (HRPTEpC), purchased from Sigma‐Aldrich (Italy), were grown in REGM medium, as indicated by the manufacturer; only cells between first and third passages were used.

Techniques: Incubation, Cell Culture, Radioactivity